The two companies last year inked an exclusive agreement, the financial details of which have not been disclosed, to design, optimize, and experimentally validate every primer pair for nearly all the protein-coding genes that make up the human transcriptome.Īll of the assays were designed following strict guidelines on maximum transcript coverage, minimal overlap with known SNPs, and spanning large introns, where possible, according to Bio-Rad.įirst published in 2009 in the journal Clinical Chemistry, the MIQE guidelines were designed to provide researchers with a roadmap for improving the quality and reliability of their qPCR data. The new PrimePCR assays are the fruits of more than a year's worth of labor by scientists at Bio-Rad and Belgian qPCR and bioinformatics firm Biogazelle. The company expects the assays, which cover approximately 95 percent of the human transcriptome, to save researchers time and resources by obviating the need to validate assays in their own laboratories. With the launch, Bio-Rad claims to be the only vendor whose entire portfolio of qPCR assays has been validated to such standards. Advances in Clinical Genomics Profilingīio-Rad this week introduced qPCR assays and panels that have been wet-lab validated to meet the minimum information for publication of quantitative real-time PCR experiments, or MIQE, guidelines.
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